Force-feedback high-speed atomic force microscope for studying large biological systems.
نویسندگان
چکیده
We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.
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References 1. Soltani Bozchalooi, I., Youcef-Toumi, K., Burns, D. J. & Fantner, G. E. Compensator design for improved counterbalancing in high speed atomic force microscopy. The Review of scientific instruments 82(11), 113712 (2011). 2. Soltani Bozchalooi, I. & Youcef-Toumi, K. Multi-actuation and PI control: a simple recipe for high-speed and large-range atomic force microscopy. Ultramicroscop...
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ورودعنوان ژورنال:
- Micron
دوره 43 12 شماره
صفحات -
تاریخ انتشار 2012